Phosphorus-containing polymers for optical signal transducers

ABSTRACT

The invention relates to a phosphorus-containing polymer for coating dielectric materials, to processes for its preparation and to its use, as well as to an optical signal transducer having a coating of the polymer and to its use.

The invention relates to a phosphorus-containing polymer for coatingdielectric materials, to processes for its preparation and to its use,as well as to an optical signal transducer having a coating of thepolymer and to its use.

Dielectric materials are coated with multifunctional polymers for bio-and chemofunctionalization, i.e. to make it possible for chemical and/orbiochemical ((bio-)chemical) recognition elements, e.g. receptors,antibodies, DNA etc., to be immobilized on their surface. Such coateddielectric materials, e.g. coated optical waveguides, are employed assignal transducers such as are used in sensor technology for bio- orchemosensors.

“Bio- or chemosensors” describes devices which, with the aid of a signaltransducer and a recognition reaction, can detect an analytequalitatively or quantitatively. “Recognition reaction” describes quitegenerally the specific binding or reaction of a so-called analyteto/with a so-called recognition element. Examples of recognitionreactions are the binding of ligands to complexes, the sequestration ofions, the binding of ligands to (biological) receptors, membranereceptors or ion channels, of antigens or haptens to antibodies, ofsubstrates to enzymes, of DNA or RNA to specific proteins, thehybridization of DNA/RNA/PNA or the processing of substrates by enzymes.Analytes may be: ions, proteins, natural or artificial antigens orhaptens, hormones, cytokines, mono- and oligosaccharides, metabolicproducts, or other biochemical markers that are used in diagnosis,ensign substrates, DNA, RNA, PNA, potential active agents, medicaments,cells, viruses. Examples of recognition elements are: sequestrants formetals/metal ions, cyclodextrins, crown ethers, antibodies, antibodyfragments, anticalines¹, enzymes, DNA, RNA, PNA, DNA/RNA-bindingproteins, enzymes, receptors, membrane receptors, ion channels, celladhesion proteins, gangliosides, mono- or oligosaccharides.

These bio- or chemosensors can be used in environmental analysis, thefood industry, human and veterinary diagnosis and crop protection, inorder to determine analytes qualitatively and/or quantitatively. Thespecificity of the recognition reaction also makes it possible todetermine analytes qualitatively or quantitatively in complex samples,e.g. ambient air, contaminated water or bodily fluids without, or onlywith minor, preliminary purification. Furthermore, bio- or chemosensorscan also be used in (bio-)chemical research and active agent testing, inorder to study the interaction between two different substances (e.g.between proteins, DNA, RNA, or biologically active substances andproteins, DNA, RNA etc.).

The recognition reaction can be integrated with the transducer, to forma bio- or chemosensor, by immobilizing the recognition element or theanalyte on the surface of the signal transducer. The recognitionreaction, i.e. the binding or reaction of the analyte with therecognition element, changes the optical properties of the mediumdirectly at the surface of the signal transducer (e.g. change in theoptical refractive index, absorption, fluorescence, phosphorescence,luminescence etc.), and this is converted into a measurement signal bythe signal transducer.

Optical waveguides are a class of signal transducers with which thechange in the optical properties of a medium that adjoins a wave-guidinglayer, typically a dielectric, can be detected. If light is conducted asa guided mode in the wave-guiding layer, the light field does notterminate abruptly at the medium/waveguide interface, but instead decaysexponentially in the so-called detection medium adjoining the waveguide.This exponentially decaying light field is referred to as an evanescentfield. If use is made of very thin waveguides whose refractive indexdiffers as much as possible from that of the adjoining medium, decaylengths of the evanescent field (intensity decreases to the value 1/e)<200 nm are achieved. If the optical properties of the medium (e.g.change in the optical refractive index^(2,3), luminescence^(4,5,6) etc.)adjoining the medium change within the evanescent field, this can bedetected via a suitable measurement layout. In this case, it is crucialfor the use of waveguides as signal transducers in bio- or chemosensorsthat the change in the optical properties of the medium be detected onlyvery close to the surface of the waveguide. Specifically, if therecognition element or the analyte is immobilized on the surface of thewaveguide, the binding to the recognition element or the reaction of therecognition element can be detected in a surface-sensitive way when theoptical properties of the detection medium (liquid, solid, gas) changeat the interface with the waveguide.

When optical waveguides are being used as bio- or chemosensors, thewaveguide/detection-medium interface is subject to stringentrequirements:

-   -   The waveguide/detection-medium interface must be stable under        the reaction conditions of the recognition reaction.    -   The recognition elements must be immobilized within the range of        the evanescent field of the waveguide.    -   The immobilization of the recognition element must be stable        under the reaction conditions of the recognition reaction.    -   The functionality of the recognition elements must still be        present after the immobilization.    -   So that only the specific recognition reaction is detected by        the signal transducer, any kind of non-specific binding to the        waveguide/detection-medium interface must be suppressed.

Recognition elements can be immobilized on the surface of waveguides ina wide variety of ways. This may be done e.g. by physisorption of therecognition elements on the signal-transducer surface. Clerc and Lukosz⁷describe the physisorption of avidin on SiO₂—TiO₂ waveguide surfaces. Ina second step, by utilizing the high-affinity avidin-biotin binding,biotinylated antibodies can be immobilized on the avidin layers appliedin this way. A disadvantage with this method of immobilizing recognitionelements on waveguide surfaces is the instability of the physisorbedavidin layer. A change in the reaction conditions, e.g. temperaturechanges, pH changes, addition of detergents etc., can cause desorptionof the avidin layer and therefore of the antibody as well.

The recognition elements may also be covalently bonded to the surface ofa waveguide. One possibility for this is provided by bifunctionalsilanes, which form a covalent bond with the waveguide surface⁸. Therecognition elements, e.g. proteins or DNA⁹, can then be covalentlybonded via a second functional group in this silane. These bifunctionalsilanes are highly reactive and the covalent bonding to the waveguidesurface must be carried out under strictly dry reaction conditions inorder to avoid hydrolysis of the reactive silane. The bonding of therecognition elements, via these silanes, to the waveguide surfaces isstable under acidic, neutral and mildly alkaline conditions. At pHvalues above 9, however, hydrolysis of the silanes can take place, whichmay cause desorption of the recognition elements from the surface.Another disadvantage with this immobilization method involves therelatively high non-specific adsorption of proteins, e.g. albumin, ontothe waveguide surfaces functionalized in this way¹⁰. The non-specificbinding to these waveguide surfaces can be reduced if blocking agents,e.g. polyethylene glycols¹¹, are bound to the surface in a second stepafter the binding of the recognition elements.

As an alternative, the binding of hydrophilic polymers, e.g.polyacrylamides, dextrans, polyethylene glycols etc. to previouslysilanised waveguide surfaces is described¹². The purpose of thesepolymers is to minimize the non-specific binding of proteins etc. to thesurface. The recognition elements are then covalently bonded to thesepolymers in a further step. A problem with this surfacefunctionalization is that several steps need to be carried out forimmobilizing the recognition elements on the surface, and that thebinding of the silanes to the waveguide surfaces is unstable at pH>9.

The recognition elements can also be bonded to polymers which, withoutprior silanisation, are applied directly to the waveguide layers.Charged copolymers based on polylysine and polyethylene glycol areadsorbed electrostatically onto some metal oxide surfaces¹³, such asTiO₂, Si_(0.4)Ti_(0.6)O₂ and Nb₂O₅. By using optical waveguides, it canbe shown that these polymers minimize the non-specific binding ofproteins to the waveguide surfaces. Use of these copolymers in biosensortechnology is discussed by the authors. A disadvantage of this methodinvolves the instability of these layers at pH values below 3 and above9, and at high salt concentrations, since, under these conditions, theelectrostatically bound polymer is desorbed from the surface.

Polymers which are either derivatized with photoactivatable groups¹⁴ orare incubated together with photocrosslinkers^(15,16) can, byphotoreaction, be applied directly to the waveguide surface andcrosslinked with one another. These polymer layers exhibit lownon-specific adsorption of proteins and are stable over a wide range ofreaction conditions. The recognition elements can be bound either duringthe photoreaction or after the photoreaction. For the latterimmobilization, use is made of polymers which, besides the photoreactivegroups, also carry functional groups which permit covalentimmobilization of the recognition elements. The photoreactive compoundsneed to be applied to the surface either by spotter or spin coating andconcentrated or dried thereon. This can lead to partial dewetting of thewaveguide surface, which results in incomplete coverage.

The coating of Ta₂O₅ waveguide surfaces with long-chain alkyl phosphatesof the general formula H₂O₃P—O—(CH₂)_(n)—CH₃ is described in thescientific literature¹⁷. It can be shown that these long-chainphosphates form tightly packed monolayers (so-called self-assembledmonolayers) on the surface of the waveguides. Without furtherpresentation of experiments, it has been proposed to useω-functionalized long-chain alkyl phosphates in biosensor technology, inwhich case the functional group in the ω position is intended to pointaway from the waveguide surface and recognition elements could be boundvia this functional group.

U.S. Pat. No. 4,904,634¹⁸ describes an active material which can be usedas an adsorbent. This material consists of a metal oxide/hydroxidesurface and a monolayer of a phosphorus-containing organic materialchemically bound to it. The phosphorus-containing organic material isfurther specified therein as follows:

-   -   the organic material has 1–2 phosphorus-containing groups.    -   the phosphorus-containing groups have the general formula        RPO(OH)₂ or RR′PO(OH), where R comprises a group containing 1 to        30 carbon atoms and R′ comprises either hydrogen or a group        containing 1 to 30 carbon atoms.    -   R and R′ may also comprise an organic radical from the group of        long- or short-chain aliphatic hydrocarbons, aromatic        hydrocarbons, carboxylic acids, aldehydes, ketones, amines,        amides, thioamides, imides, lactams, anilines, pyridines,        piperidines, carbohydrates, esters, lactones, ethers, alkenes,        alkynes, alcohols, nitriles, oximes, organosilicones, ureas,        thioureas, perfluoro compounds (organic), perchloro compounds,        perbromo compounds and combinations of these groups.    -   R or R′ may also have a functional group at a position in the        molecule which is spaced apart from the phosphorus-containing        group. The functional group may be a carboxyl, glucose, cyano,        cyanate, isocyanate, thiocyanate, phenyl, diphenyl, tertiary        butyl, sulphonic acid, benzylsulphonic, halogen, nitrate,        phosphate, phosphinate, phosphinite, phosphonate,        hydroxymethylamide, alkoxymethylamide, benzophenone, azide,        triazene, acylphosphane, quaternary ammonium group or        combinations of these groups.    -   R or R′ may also carry a cation exchange group, such as —HSO₃,        —N(CH₃)₃Cl, —COONa, —NH₂ and —CN.    -   R may also be an oligomer which is made up of 2–4 monomers and        has a molar mass <2000 g/mol.

As an application, reference is made to an active material which issuitable as an adsorbent. Other applications which are mentioned are:

-   -   Support material for chromatography.    -   Ion exchange material.    -   Coupling element for biological material, such as enzymes,        antibodies, cells, yeasts, proteins, microbes, pharmaceuticals,        vaccines.    -   Coating of piezoelectric crystals.    -   Coatings for passivation of biological implants (bones etc.).    -   Additives of medicinal products.

In the Patent Application GB 2 221 466¹⁹, the same author describesbiologically active particles which are made up of a metaloxide/hydroxide core whose surface has functionalized organophosphoruscompounds, such as described in U.S. Pat. No. 4,904,634, bound to it.The patent relates exclusively to biologically active particles.

U.S. Pat. No. 4,308,079²⁰ describes corrosion inhibitors for aluminiumoxide surfaces. As inhibitors, use is made of aminophosphonates whichhave the following general structure: NR₃, NHR₂, R′NR₂, (CH₂NR₂)₂, andR₂NCH₂CH₂NRCH₂CH₂NR₂, wherein R is CH₂PO(OH)₂ and R′ is an alkyl chaingroup having from 1 to 5 carbon atoms. Other applications are notdescribed.

Polyoxyalkylene diphosphonates, which can be used to improve thedispersion of calcium carbonate²¹ and magnetite nanoparticles²², aredescribed in the scientific literature. Polymers of the structureH—(OCH₂CH₂)_(n)—N(CH₃)—CH₂—PO₃H₂ and H—(OCH₂CH₂)_(n)—N(CH₂PO₃H₂)₂ with20<n<70, which build up a polymer layer on the surface of thenanoparticles, are used for this purpose. If these polymers are actuallyadded during the synthesis of the nanoparticles, a narrow sizedistribution is observed. A further chemical modification, as well asbinding of biologically active agents to these polymer-coatednanoparticles, is discussed.

Apart from the disadvantages, described in the prior art, which need tobe avoided, the waveguide/detection-medium interface is subject tostringent requirements when optical waveguides are being used as bio- orchemosensors:

-   -   The waveguide/detection-medium interface must be stable under        the reaction conditions of the recognition reaction.    -   Recognition elements must be immobilized within the range of the        evanescent field of the waveguide.    -   The immobilization of the recognition elements must be stable        under the reaction conditions of the recognition reaction.    -   The functionality of the recognition elements must still be        present after the immobilization.    -   So that only the specific recognition reaction is detected by        the signal transducer, any kind of non-specific binding of the        elements today recognized to the waveguide/detection-medium        interface must be suppressed.

The invention relates to a phosphorus-containing polymer, suitable forcoating dielectric surfaces, of the general formula I or II,P(A)_(m)(F)_(n1)(U)_(o1)  (I)P(A)_(m)(UF_(n2))_(o2)  (II)in which

P stands for a linear or branched, uncrosslinked or crosslinked, homoorheteropolymeric polymer component,

A stands for identical or different phosphorus-containing groups bondedto P,

m stands for a number from 3 to approximately 1000,

F stands for identical or different functional groups bonded directly orindirectly to P, which are present in addition to A,

n1 stands for a number from 1 to approximately 1000,

n2 stands for a number from 1 to approximately 100,

U stands for identical or different, linear or branched, uncrosslinkedor crosslinked oligomeric or polymeric segments, made up of identical ordifferent monomers, which are bonded to P,

o1 stands for a number from 0 to approximately 1000,

o2 stands for a number from 1 to approximately 1000.

The polymer according to the invention is suitable for coatingdielectric materials, in particular dielectric waveguide surfaces. Thethickness of the coating is usually between 0.5 and 700 nm, preferablybetween 0.5 and 200 nm, in particular between 0.5 and 10 nm.

The invention secondly relates to a process for preparing a polymeraccording to the invention by copolymerizing

(A) a monomer containing a phosphorus-containing group A, or a pluralityof identical or different monomers containing identical or differentphosphorus-containing groups A with

(B) a monomer containing a functional group F, or a plurality ofidentical or different monomers containing identical or differentfunctional groups F, and

(C) optionally, a monomer containing a segment U, or a plurality ofidentical or different monomers containing identical or differentsegments U, to form a polymer of the formula I,

or with

(B) a monomer containing a unit (UF_(n2))_(o2) according to formula II,or a plurality of identical or different monomers containing identicalor different units of the formula (UF_(n2))_(o2) according to formulaII,

to form a polymer of the formula II.

The invention thirdly relates to a process for preparing a polymeraccording to the invention by

(i) preparing a polymer, which forms the polymer component P and carriesidentical or different functional groups that are suitable as functionalgroups F, preferably hydroxyl groups, carboxyl groups, derivatives ofcarboxyl groups and/or amine groups,

(ii) reacting some of the functional groups to form identical ordifferent phosphorus-containing groups A, and

(iii) optionally, reacting some of the functional groups to formidentical or different segments U,

wherein step (iii) can be carried out after, before or together withstep (ii), and wherein not all the functional groups are converted insteps (ii) and (iii), and the unreacted functional groups form thefunctional groups F of the polymer. In this case, some or all of thefunctional groups that have not been converted in steps (ii) and (iii)may be reacted with one or more identical or different crosslinkers toform functional groups F.

The invention fourthly relates to the use of a polymer according to theinvention for coating dielectric materials, in particular dielectricwaveguides. In this case, the polymer may be used for coating dielectricmaterials, in particular dielectric waveguides, made of TiO₂, Ta₂O₅,ZrO₂, HfO₂ or Al₂O₃, preferably of TiO₂ or Ta₂O₅.

The invention fifthly relates to an optical signal transducer having acoated dielectric waveguide, whose coating consists of a polymeraccording to the invention.

The invention sixthly relates to the use of an optical signal transducerhaving a coated dielectric waveguide according to the invention forimmobilizing chemical and/or biochemical recognition elements.

The phosphorus-containing polymer according to the invention containsvarious functional groups or segments for satisfying the saidrequirements to which the waveguide coating is subject:

The polymer component P.

The phosphorus-containing groups A of the polymer, which ensure stablebinding of the polymer to the surface of the waveguide. In this case,preferably between 0.001 and 10 milliequivalents (mEq) ofphosphorus-containing groups are present per gram of polymer, inparticular from 0.01 to 5 mEq/g, particularly preferably from 0.1 to 3mEq/g.

-   -   The functional groups F of the polymer, via which the        recognition elements can be immobilized directly or with the aid        of a crosslinker covalently, coordinatively or via another        chemical bond onto the polymer, and therefore onto the surface        of the bio- or chemosensor. In this case, preferably between        0.001 and 20 milliequivalents (mEq) of functional groups are        present per gram of polymer, in particular from 0.01 to 10        mEq/g, particularly preferably from 0.5 to 10 mEq/g.    -   The segments U, which suppress the non-specific binding of        proteins etc. to the polymer, and therefore to the waveguide. U        may be omitted from the polymer if the suppression of the        non-specific binding is already achieved by the polymer        component. In this case, preferably between 0.001 and 20        milliequivalents (mEq) of segments U are present per gram of        polymer, in particular from 0.01 to 10 mEq/g, particularly        preferably from 0.5 to 10 mEq/g.

The polymers according to the invention may be linear, branched orcrosslinked, and have an average molar mass of from 1000 to 10,000,000g/mol, preferably from 2100 to 1,000,000 g/mol, particularly preferablyfrom 5000 to 500,000 g/mol, most preferably from 5000 to 300,000 g/mol,in particular from 10,000 to 150,000 g/mol. The molar mass may bedetermined e.g. by vapourpressure osmosis or light scattering.

Polymer component P

The polymer components P may be made up statistically or in blockfashion. They are typically hydrophilic polymers, the term “hydrophilicpolymer” describing, in the scope of the teaching of the invention, apolymer which can be wetted or made to swell with water or aqueoussolutions. Examples include:

-   -   Polyvinyl alcohols, polyvinyl amine, polyallyl amine,        polyethylene imine, polyacrylates, polyacrylamides, imides of        polymaleic anhydride-alt-methyl vinyl ether or derivatives        thereof.    -   Linear polyethylene glycols, polypropylene glycols or        derivatives thereof.    -   Branched or star-branched polyethylene glycols, as described        e.g. in U.S. Pat. No. 5,171,264²³, to which reference is made in        this regard and whose content is hereby included in this        application, or derivatives thereof.    -   Polyureas, polyurethanes, polyesters, polycarbonates,        polyhydroxycarboxylic acids, or derivatives thereof which are        made up of diols/polyols and/or diamines/polyamines. The        diols/polyols may be polyethylene glycols, polypropylene glycols        etc. The diamines/polyamines may be jeffamine, polyethylene        imines, polyvinyl amine, polyallyl amine, polyethylene imine,        etc.    -   Polysaccharides such as cellulose, starch, agarose, dextran,        chitosan, hyaluronic acid or derivatives thereof, in particular        hydroxyalkyl derivatives or acid semiesters.    -   Polypeptides or derivatives thereof which are made up of one or        more various amino acids, e.g. polylysine,        polyphenylalanine-lysine, polyglutamates,        polymethylglutamate-glutamate, polyphenylalanine-glutamate,        polyserine, polyglycine, polyserine-glycerine etc.    -   Branched polyols based on glycidol, such as e.g. in Patent        Applications EP 0 116 978 and WO 00/37532, to both of which        reference is made in this regard and whose content is hereby        included in this application, or derivatives thereof. Preferred        are polyols based on glycidol with a degree of polymerization of        1 to 300, a polydispersity index below 1.7, a content of        branched units, based on the sum of all monomeric units and        determined by ¹³C NMR spectroscopy, of 10 to 33 mol %.

Phosphorus-containing groups A

Stable anchoring of the polymer on the waveguide surface is achievedthrough several phosphorus-containing groups which are bonded directly,or via a spacer S, to a carbon atom of the polymer component.

The groups A preferably satisfy the formulaA =S_(s)Y_(p)in which

p stands for the number 1 and

s stands for the number 0 (i.e. A=Y) or 1 (i.e. A=SY) or

p stands for the numbers 2, 3, 4, 5 or 6 and

s stands for the number 1 (i.e. A=SY_(p))

and in which the group or groups Y is/are selected from the followingphosphorus-containing radicals:

—O(R′O)PO₂H, —P(R′O)O₂H, —N(CH₂—P(R′O)O₂H)₂, —N(R′)—CH₂—P (R′O)O₂H,—CH(P(R′O)O₂H)N(CH₂—P(R′O)O₂H)₂, —CH(CH₂—P(R′O)O₂H)₂,—CR′(CH₂—P(R′O)O₂H)₂, —C(CH₂—P(R′O)O₂H)₃,

where R′ stands for —H, —CH₃ or —C₂H₅.

The polymer preferably contains one or more of the following groups Y:

—O(R′O)PO₂H, —P(R′)O₂H, —N(CH₂—P(R′O)O₂H)₂, in particular—N(CH₂—P(R′O)O₂H)₂,

where R′ preferably stands for —H.

The spacer S is directly coupled to a C atom of the polymer and carriesp identical or different phosphorus-containing radicals Y. According tothe invention, the following spacers are preferred (group(s) Y are alsoindicated):

—(CH₂)_(q)—(O—CH₂—CH₂)_(r)—Y, —(CH₂)_(q)—(O—CH₂—CH₂—CH₂)_(r)—Y,—(CH₂)_(q)—(O—CH₂—CH₂)_(r)—C₆H₄Y, —(CH₂)_(q)—(O—CH₂—CH₂)_(r)—C₆H₃Y₂,—(CH₂)_(q)—(O—CH₂—CH₂)_(r)—C₆H₂Y₃,

where q stands for numbers from 0 to 20 and r stands for numbers from 0to 100.

In a particular embodiment, the polymer according to the inventioncontains phosphorus-containing groups A in the form of a spacer Scarrying from one to six identical or different phosphorus-containingradicals.

The following groups A, which are coupled directly to a C atom of thepolymer, are preferred:

—PO₃H₂, —NH—CH₂—CH₂—PO₃H₂, —CH₂—N(CH₂—PO₃H₂)₂, —N(CH₂—PO₃H₂)₂,—(CH₂)₄N(CH₂—PO₃H₂)₂, —OPO₃H₂.

Functional groups F for immobilizing recognition elements F stands forfunctional groups which are bonded directly to a carbon atom of thepolymer, and via which recognition elements can be immobilized directlyor with the aid of a crosslinker covalently, coordinatively or viaanother chemical bond onto the polymer, and therefore onto the surfaceof the bio- or chemosensor. The direct coupling of the recognitionelements can be carried out before the waveguide is coated with thepolymer, or after this. Typical functional groups for covalentlyimmobilizing recognition elements are e.g.: carboxylic acid, carboxylicacid ester, carboxylic acid chloride, carboxylic acid anhydride,carboxylic acid nitrophenyl ester, carboxylic acid N-hydroxysuccinimide,carboxylic acid imidazolide, carboxylic acid pentafluorophenyl ester,hydroxyl, toluenesulphonyl, trifluoromethylsulphonyl, epoxy, aldehyde,ketone, β-dicarbonyl, isocyanate, thioisocyanate, nitrile, amine,aziridine, hydrazine, hydrazide, nitro, thiol, disulphide, thiosulphite,halogen, iodoacetamide, bromoacetamide, chloroacetamide, boric acidester, maleimide, α,β-unsaturated carbonyls, phosphate, phosphonate,hydroxymethylamide, alkoxymethylamide, benzophenone, azide, triazene,acylphosphane.

Alternatively, the recognition elements may also be coordinativelyimmobilized onto the polymer. Typical groups for this are, for example:iminodiacetic acid, nitrilotriacetic acid.

Alternatively, biochemical recognition reactions may be used toimmobilize recognition elements onto the polymer. To that end, thefollowing groups may be bonded to the polymer: StrepTag²⁴, digoxin,digoxigenin, biotin, thiobiotin, fluorescein, dinitrophenol,streptavidin, avidin, etc.

In a particular embodiment, the polymer according to the inventioncontains functional groups F with crosslinkers, which may be bonded tothe polymer before or after the waveguides are coated. Thesecrosslinkers may be linear, branched or crosslinked molecules, oligomersor polymers having a molar mass, or average molar mass, of from 50 to50,000, which carry two or more identical or different functionalgroups, or other commercial crosslinkers. Preferred crosslinkers can bedescribed generally by the formulaP1(F1)_(m)(F2)_(n)

with m, n=0, 1, 2, . . . 100, preferably with m+n≧2, preferably with m,n=1, 2 or 3.

P1 maybe:

-   -   Linear or branched alkyl or aryl radicals having 1–10 C atoms.    -   Linear polyethylene glycols, polypropylene glycols, copolymers        of these polymers or derivatives thereof.    -   Branched or star-branched polyethylene glycols, as described        e.g. in U.S. Pat. No. 5,171,264²⁵, to which reference is made in        this regard and whose content is hereby included in this        application, or derivatives thereof.    -   Polysaccharides such as cellulose, starch, agarose, dextran,        chitosan, hyaluronic acid or derivatives thereof.    -   Polypeptides or derivatives thereof which are made up of one or        more various amino acids, e.g. polylysine,        polyphenylalanine-lysine, polyglutamate,        polymethylglutamate-glutamate, polyphenylalanine-glutamate,        polyserine, polyglycine, polyserine-glycerine etc.    -   Branched polyols or oligools based on glycidol, such as e.g. in        Patent Applications EP 0 116 978 and WO 00/37532, to both of        which reference is made in this regard and whose content is        hereby included in this application, or derivatives thereof.        Preferred are polyols based on glycidol with a degree of        polymerization of 1 to 300, a polydispersity index below 1.7, a        content of branched units, based on the sum of all monomeric        units and determined by ¹³C NMR spectroscopy, of 10 to 33 mol %.

F1 are functional groups which permit coupling of the crosslinker to thefunctional groups F of the polymer. F2 are functional groups to whichthe recognition elements can be bonded via a covalent, coordinative orother chemical bond. F1 and F2 may be identical or different functionalgroups. Examples of the groups F1 and F2 are the following functionalgroups:

carboxylic acid, carboxylic acid ester, carboxylic acid chloride,carboxylic acid anhydride, carboxylic acid nitrophenyl ester, carboxylicacid N-hydroxysuccinimide, carboxylic acid imidazolide, carboxylic acidpentafluorophenyl ester, hydroxyl, toluenesulphonyl,trifluoromethylsulphonyl, epoxy, aldehyde, ketone, β-dicarbonyl,isocyanate, thioisocyanate, nitrile, amine, aziridine, diazirine,hydrazine, hydrazide, nitro, thiol, dithiol, thiosulphite, halogen,iodoacetamide, bromoacetamide, chloroacetamide, boric acid ester,maleimide, α,β-unsaturated carbonyls, phosphate, phosphonate,hydroxymethylamide, alkoxymethylamide, benzophenone, azide, triazene,acylphosphane.

A preferred crosslinker of formula P1(F1)_(m)(F2)_(n) is ethylene glycolbis-succinimidyl succinate.

Segments U for suppressing non-specific binding

The polymer can have segments U which suppress the non-specific bindingof proteins etc. to the polymer, and therefore to the waveguide. Thesesegments are covalently bonded to the polymer unit P and may bepreferably hydrophilic linear, branched or crosslinked oligomers orpolymers having a preferred molar mass, or average molar mass, of from100 to 10,000. Examples of such segments are:

-   -   Linear oligo- or polyethylene glycols, oligo- or polypropylene        glycols, copolymers of these oligomers/polymers or derivatives        thereof.    -   Branched or star-branched oligo- and polyethylene glycols, as        described e.g. in U.S. Pat. No. 5,171,264²⁶, to which reference        is made in this regard and whose content is hereby included in        this application, or derivatives thereof.    -   Oligo- or polysaccharides such as cellulose, starch, agarose,        dextran, chitosan, hyaluronic acid or derivatives thereof.    -   Oligo- or polypeptides or derivatives thereof which are made up        of one or more various amino acids, e.g. polylysine,        polyphenylalanine-lysine, polyglutamate,        polymethylglutamate-glutamate, polyphenylalanine-glutamate,        polyserine, polyglycine, polyserine-glycerine etc.    -   Branched polyols or oligools based on glycidol, such as e.g. in        Patent Applications EP 0 116 978 and WO 00/37532, to both of        which reference is made in this regard and whose content is        hereby included in this application, or derivatives thereof.        Preferred are polyols based on glycidol with a degree of        polymerization of 1 to 300, a polydispersity index below 1.7, a        content of branched units, based on the sum of all monomeric        units and determined by ¹³C NMR spectroscopy, of 10 to 33 mol %.

These segments may be omitted from the polymer if the suppression of thenon-specific binding is already achieved by the polymer component.

Preparation of the polymer

The polymer according to the invention can be prepared e.g. bycopolymerizing various monomers that contain the groups A, F and U,using processes which are known to an experienced chemical synthesist.For instance, the polymer may be prepared e.g. by copolymerizingvinylphosphonic acid, polyethylene glycol methyl ether acrylate andacrylic acid. The recognition element may then be bound before or afterthe polymer is applied to the waveguide surface. To that end, thecarboxylic acid groups are activated by reaction, e.g. withcarbodiimides, and then reacted with nucleophilic functional groups ofthe recognition element, which leads to covalent bonding of therecognition element to the polymer.

Alternatively, however, known processes may be used to synthesizepolymers which have identical or different functional groups F. Thephosphoruscontaining groups A and, optionally, segments U may then beintroduced in further steps. In this case, it is necessary to ensurethat only a certain proportion of the groups F are converted. Therecognition elements can then be bonded via the remaining groups F. Inthe case of polymers which carry e.g. hydroxyl groups as functionalgroups F, the phosphorus-containing groups A may be produced by reactionwith polyphosphoric acid. In this case, only some of the hydroxyl groupsare converted. The recognition element may then be bound before or afterthe polymer is applied to the waveguide surface. To that end, thehydroxyl groups are activated by reaction, e.g. with toluenesulphonylchloride, and then reacted with nucleophilic functional groups of therecognition element, which leads to covalent bonding of the recognitionelement to the polymer.

The polymer may also be prepared e.g. from polymers which carry thecarboxylic acid groups or derivatives thereof The phosphorus-containinggroups A are introduced by reaction e.g. with aminoethylphosphonic acidor H₂N—(C₆H₄)₂—N (CH₂PO₃H₂)₂. In this case, only some of the carboxylicacid groups are converted. The recognition element may then be boundbefore or after the polymer is applied to the waveguide surface. To thatend, the carboxylic acid groups are activated by reaction, e.g. withcarbodiimides, and then reacted with nucleophilic functional groups ofthe recognition element, which leads to covalent bonding of therecognition element to the polymer.

If amine-containing polymers, e.g. polyethylene imine, polyvinyl amine,polyallyl amine or polylysine are reacted according to Mannich/Mödritzerwith formaldehyde and phosphoric acid, the phosphorus-containing groupsA can hence be introduced. In this case, only some of the amine groupsare converted. The recognition element may then be bound before or afterthe polymer is applied to the waveguide surface. To that end, the aminegroups are activated by reaction, e.g. with a bifunctional crosslinkersuch as ethylene glycol bis-succinimidyl succinate. In this case,activated carboxylic acid groups are introduced, to which nucleophilicgroups of the recognition element can bond.

Application of the polymer to the waveguides

The phosphorus-containing groups A are preferably suitable for anchoringthe polymer to waveguides made of materials such as TiO₂, Ta₂O₅, ZrO₂,HfO₂, Al₂O₃, SiO₂ (Si(Ti)O₂), In₂O₃/SnO₂ (ITO), aluminosilicates, Nb₂O₅,vanadium oxides, or mixtures of these materials. The waveguide materialsmay, however, also be oxides or hydroxides of the following elementsthat can form oxides or hydroxides: Sc, Y, Ti, Zr, Hf; V, Nb, Ta, Cr,Mo, W, Mn, Tc, Re, Fe, Ru, Os, Co, Rh, Ir, Ni, Pd, Pt, Cu, Ag, Au, Zn,Cd, Hg, B, Al, Ga, In, Tl, Ge, Sn, Pb, As, Sb, Bi, lanthanides, actingnights and mixtures thereof, as well as mixtures of group Ila (Be, Mg,Ca, Sr, Ba, Ra) and VIb (Se, Te, Po).

The polymer is applied to the waveguide surfaces from organic or aqueoussolution. This may be done by incubation in the solution, immersion,spraying, spotting, spin coating or similar standard processes.Typically, solutions of between 0.001 and 1000 g/l, in particularbetween 0.1 and 10 g/l, are used and the waveguide surfaces are coatedat temperatures of between 0 and 200° C., in particular between 20 and30° C. The incubation time of the waveguide materials with the polymersolutions may be between 10 s and 48 h, typically between 10 min and 24h. After the incubation, the waveguides are washed with organic solventsor aqueous solutions and, optionally, derivatized further.

Immobilization of the recognition elements on the polymer Recognitionelements can be immobilized directly or with the aid of a crosslinkercovalently, coordinatively or via another chemical bond onto thefunctional groups F of the polymer, and therefore onto the surface ofthe bio- or chemosensor. The direct coupling of the recognition elementscan be carried out before the waveguide is coated with the polymer, orafter this. The recognition elements may be covalently bonded to thefunctional groups F via their own functional groups, such as carboxylicacid, carboxylic acid ester, carboxylic acid chloride, carboxylic acidanhydride, carboxylic acid nitrophenyl ester, carboxylic acidN-hydroxysuccinimide, carboxylic acid imidazolide, carboxylic acidpentafluorophenyl ester, hydroxyl, toluenesulphonyl,trifluoromethylsulphonyl, epoxy, aldehyde, ketone, β-dicarbonyl,isocyanate, thioisocyanate, nitrile, amine, aziridine, hydrazine,hydrazide, nitro, thiol, disulphide, thiosulphite, halogen,iodoacetamide, bromoacetamide, chloroacetamide, boric acid ester,maleimide, α,β-unsaturated carbonyls, phosphate, phosphonate,hydroxymethylamide, alkoxymethylamide, benzophenone, azide, triazene,acylphosphane. The combination of which functional group of therecognition element reacts with which functional group of the polymerresults from the possibilities, which are known to chemists, forreaction between the functional groups.

Proteins as recognition elements can be immobilized on the polymer e.g.via their amino acid side chains. Especially amino acids, e.g. lysines,cysteines, serines, tyrosines, histidines, glutamates, aspartates, whichare localized on the surface of a protein, have functional groups intheir side chains which can form a covalent bond with the functionalgroups of the polymer. Functional groups can also be produced in therecognition elements derivatization (phosphorylation of tyrosines),oxidation (e.g. oxidation of diol units of glycosylated proteins to formaldehyde groups), reduction (e.g. of disulphide bridges to form thiols)or coupling of a crosslinker.

Besides covalent immobilization of the recognition elements onto thepolymer, the recognition elements may also be coordinatively bonded tothe polymer. For example, proteins such as enzymes, antibody fragmentsand receptors with special affinity sequences, e.g. the6xhistidine-tag²⁷ can be prepared using methods of molecular biology.These affinity sequences have a high affinity and specificity for metalion complexes, e.g. nickel nitrilotriacetic acid, copper iminodiaceticacid, which may be introduced into the polymer as a functional group F.

Alternatively, biochemical recognition reactions may also be used toimmobilize recognition elements onto the polymer. The very specific,high-affinity binding of biotin to streptavidin²⁸ can be used toimmobilize recognition elements onto the polymer. To that end, thefunctional groups F of the polymer must be e.g. streptavidin. Therecognition element is then functionalized with biotin and can hence bebonded to the polymer. Alternatively, the recognition element may beprovided by molecular biological or chemical means with a short aminoacid sequence, the so-called StrepTag²⁴, which also has a highspecificity and affinity for streptavidin.

Advantages

Multifunctional polymers for bio- and chemofunctionalization chemisorbfrom organic or aqueous solution onto waveguide surfaces. Owing to thespecific binding of the phosphorus-containing groups to waveguidematerials, they form a stable layer on the waveguide. The binding isstable over a wide pH range (pH=1 to pH=14), temperature range (0° C. to100° C.) as well as at high salt concentrations (1M). Even the presenceof detergents in the reactions solution does not cause desorption of thepolymer from the waveguide surface. Only a monolayer of polymer can beapplied on the surface, fully in keeping with chemisorption, since thepolymer is bound specifically to the waveguide surface via thephosphorus-containing groups. The thickness of the polymer layers istherefore self-limiting, and can be adjusted in a controlled way throughthe average molar mass and the chemical structure of the polymer. It istherefore possible to ensure that the recognition elements areimmobilized within the evanescent light field, and therefore in thesensitive detection range of the signal transducer.

Special segments of the polymer, or the polymer itself, very effectivelyprevent the non-specific binding of proteins and other organic as wellas inorganic compounds to the waveguide surfaces. This makes it possibleto detect very specifically only the desired recognition reaction withthe aid of the signal transducer. Therefore, both the specificity of thesensor and the signal-to-noise ratio are improved significantly.

The recognition elements are bound stably to the polymer via covalent,coordinative or other chemical bonds. Desorption of the recognitionelements from the polymer is hence avoided. A further effect of the verylow non-specific interaction of the polymer with proteins and otherorganic molecules is that the immobilized recognition elements have ahigh activity. The recognition elements are bound very specifically tothe polymer, and further non-specific interactions of the recognitionelements with the polymer, which could reduce the activity of therecognition elements, do not occur, or occur only to a very smallextent.

Use

The polymer can be applied to a very wide variety of waveguidematerials. Recognition elements can then be immobilized onto the polymerwhile preserving their activity. The polymer hence acts as an interfacefor immobilizing recognition elements on signal transducers, e.g.waveguides. The polymer therefore permits the integration of arecognition reaction and a signal transducer to form a sensor. Owing tothe flexible concept of the polymer, it is possible to immobilize verydisparate recognition elements, so that the sensor can be used inenvironmental analysis, the food industry, human and veterinarydiagnosis and crop protection, in order to determine analytesqualitatively and/or quantitatively. Since the polymer prevents thenon-specific binding of organic, inorganic compounds and macromoleculesto the sensor surface, it is also possible to determine analytesqualitatively or quantitatively in complex samples, e.g. ambient air,contaminated water or bodily fluids without, or only with minor,preliminary purification.

Furthermore, the polymer can also be used in (bio-)chemical research andactive agent testing, in order to study the interaction between twodifferent substances in parallel or sequentially by means of a suitablesignal transducer. It is hence possible to study e.g. the interaction ofbiologically active substances, e.g. potential active agents, withbiomolecules such as proteins, membrane receptors, ion channels, DNA,RNA etc.).

Literature

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EXAMPLES Example 1

Polymer Made from Phosphonate-functional Copolymers.

A mixture of 50 g of N-methyl-2-pyrrolidone (NMP), 5 g ofvinylphosphonic acid, 10 g of triethylamine, 15 g of methacryloxyethylacetoacetate, 30 g of polyethylene glycol methyl ether acrylate (molarmass 750 g/mol), 0.5 g of azobisisobutyronitrile and 1.5 g of dodecylmercaptan was heated for 6 h to 65° C. After cooling, the solution wasadjusted in ethanol to a concentration of 0.1 mg of polymer per ml ofsolution, and the waveguide surfaces were incubated in this solution for18 h. The waveguides were then washed with ethanol and 10 mM (M=mol/l)NaOH. A solution of 2 mg/ml of anti-myoglobin monoclonal mouseantibodies in 10 mM sodium acetate buffer, adjusted to pH=5, wasprepared and the waveguide surfaces were incubated in it for 2 h. A 1.5ng/mm² surface concentration of antibodies was obtained.

Example 2

Polymer Made from Phosphate Esters of Polyvinyl Alcohol.

A mixture of 50 g of a 10% strength solution of polyvinyl alcohol(polyvinyl acetate with an 88% degree of saponification and a Höpplerviscosity of 18 for the 4% strength solution in water) in DMSO and 0.1%of polyphosphoric acid was heated for 15 min to 100° C. After cooling,10 g of succinic anhydride were added to the solution and stirred at 21°C. for 3 h. In the next step, the solution was adjusted in ethanol to aconcentration of 1 mg of polymer per ml of solution, and the waveguidesurfaces were incubated in this solution for 18 h. The waveguides werethen washed with ethanol and 10 mM NaOH. The surface was incubated for10 min in a solution of 1 M N-hydroxysuccinimide and 1 MN-dimethylaminopropyl-N′-ethyl-carbodiimide hydrochloride in ultrapurewater, and then washed with ultrapure water. A solution of 2 mg/ml ofanti-myoglobin monoclonal mouse antibodies in 10 mM sodium acetatebuffer, adjusted to pH=5, was prepared and the waveguide surfaces wereincubated in it for 2 h. A 2.5 ng/nu² surface concentration ofantibodies was obtained.

Example 3

Polymer Made from Imidised MSA Copolymers.

15.6 g of polymaleic anhydride-alt-methyl vinyl ether (MW (average molarmass)=216,000 g/mol) were added portionwise to a mixture of 9.5 g of2-(2-aminoethoxy)-ethanol, 1.11 g of aminomethanephosphonic acid, 1 g oftriethylamine and 100 ml of water at 70° C. After cooling, the solutionwas adjusted in ethanol to a concentration of 10 mg of polymer per ml ofsolution, and the waveguide surfaces were incubated in this solution for18 h. The waveguides were then washed with ethanol and 10 mM NaOH. Thesurfaces were incubated in a 10 mg/ml solution of ethylene glycolbissuccinimidyl succinate in DMSO for 30 min and then washed with DMSOand ultrapure water. A solution of 2 mg/ml of anti-human chorionicgonadotropin monoclonal mouse antibodies in 10 mM sodium acetate buffer,adjusted to pH=5, was prepared and the waveguide surfaces were incubatedin it for 2 h. A 2.0 ng/mm² surface concentration of antibodies wasobtained.

Example 4

Polymer Made from Phosphonate-functional Copolymers Grafted withPolyglycidol.

Preparation of the grafting basis (polyglycidol modified with fattyacid):

A mixture of 28 g of soybean oil fatty acid and 74 g of epoxypropanol(glycidol) was heated for 1 h to 140° C. and then a mixture of 0.4 g ofphosphoric acid and 333.5 g of epoxypropanol was added in portions over6 h. The mixture was then stirred for a further 16 h at 140° C.

A mixture of 20 g of the previously prepared polyglycidol modified withfatty acid, 20 g of methacryloyloxyethyl acetoacetate, 2 g ofvinylphosphonic acid, 2 g of triethylamine, 42 g of NMP and 0.4 g ofazobisisobutyronitrile was heated for 16 h to 65° C. and for 1 h to 100°C. After cooling, the solution was adjusted in ethanol to aconcentration of 3 mg of polymer per ml of solution, and the waveguidesurfaces were incubated in this solution for 10 h. The waveguides werethen washed with ethanol and 10 mM NaOH. A solution of 2 mg/ml ofantimyoglobin monoclonal mouse antibodies in 10 mM sodium acetatebuffer, adjusted to pH=5, was prepared and the waveguide surfaces wereincubated in it for 2 h. A 3.5 ng/mm² surface concentration ofantibodies was obtained.

Example 5

Polyglycidol, Derivatized with Maleic Acid Anhydride andImino-bis-methylene Phosphonic Acid.

Preparation of the thiol derivatized imido-di-methylene phosphonic acidreagent:

A mixture of 100 g of mercapto ethylamine hydrochloride, 150 gphosphonic acid and 170 g of water was heated to 100° C. and over 1 h287 g of formaldehyde (37% strength) were added dropwise. The mixturewas stirred for a further hour and then the solvent was removed undervacuum.

Preparation of the polyglycidol:

1.88 g of hexadecyl amine were melted in a 250 ml glas reactor heated to100° C. and reacted with 1.2 g glycidol. Then 0.9 ml of potassiumethoxide solution (25% strength in methanol) was added and excessivemethanol removed under vacuum. At 140° C. the residue was dissolved in15 ml of dry diglyme. At a speed of 25 ml per hour 260 g of glycidol in350 ml of dry THF were added in portions. Upon completion of theaddition the reaction mixture was dissolved in 1200 ml of methanol andneutralized by filtration over an acidic ion exchanger (Amberlite®IR-120). The filtrate was precipitated in 121 of acetone and the yieldedpolymer was dried at 80° C. for 12 h under vacuum. 254 g of a colorless,highly viscous liquid with a molar mass of 30,000 g/mol and apolydispersity of 1.23 were received. All molecules comprise theinitiator as Kerneinheit and 27% of branched building units.

Subsequently a mixture of 1 g of the previously prepared polyglycidoland 5 g of DMSO was heated to 50° C. Then 0.2 g of maleic acid anhydridewas added. After 15 min it was heated to 80° C. and 0.2 g of thiolderivatized imido-bis-methylene phosphonic acid reagent and 0.3 gtriethyl amine were added. After 15 min 0.05 g of azoisobutyro nitrilewas added and it was stirred for a further 4 h at 80° C. and then for afurther hour at 100° C.

After cooling, the solution was adjusted in ethanol to a concentrationof 2 mg of polymer per ml of solution, and the waveguide surfaces wereincubated in this solution for 16 h. The waveguides were then washedwith ethanol and water. The surface was incubated in a solution of 1 Mof N-hydroxy succinimide and 1 M of N-dimethyl aminopropyl N′-ethylcarbodiimide hydrochloride in ultrapure water for 10 min and then washedwith ultrapure water. A solution of 2 mg/ml of anti-myoglobin monoclonalmouse antibodies in 10 mM sodium acetate buffer, adjusted to pH=5, wasprepared and the waveguide surfaces were incubated in it for 2 h. A 2.8ng/mm² surface concentration of antibodies was obtained.

Example 6

Polymer Made from Dextran Modified with Acetoacetoxy and PhosphateEster.

A mixture of 10 g of dextran (MW=40,000 g/mol), 7 g of tert-butylacetoacetate, 100 g of DMSO and 0.5 g of polyphosphoric acid was heatedfor 4 h to 80° C. After cooling, the solution was adjusted in ethanol toa concentration of 1 mg of polymer per ml of solution, and the waveguidesurfaces were incubated in this solution for 8 h. The waveguides werethen washed with ethanol and 10 mM NaOH. A solution of 2 mg/ml ofstreptavidin in 10 mM sodium acetate buffer, adjusted to pH=5, wasprepared and the waveguide surfaces were incubated in it for 2 h. A 4.5ng/mm² surface concentration of streptavidin was obtained.

Example 7

Polymer Made from Phosphonate-functional Polylysine.

500 mg of poly-L-lysin hydrobromide (MW=150,000 to 300,000 g/mol), 170mg of phosphoric acid and 4 ml of water was heated to 100° C., and then324 mg of formaldehyde (37% strength) were added. The mixture wasstirred for 1 h at 100° C. After cooling, the solution was adjusted inethanol to a concentration of 1 mg of polymer per ml of solution, andthe waveguide surfaces were incubated in this solution for 2 h. Thewaveguides were then washed with ethanol and 10 mM NaOH. The surfaceswere incubated with a solution of 10 mg/ml of carboxymethyldextran(MW=15,000 g/mol), 0.1 M of N-hydroxysuccinimide and 0.1 M ofN-dimethylaminopropyl-N′-ethyl-carbodiimide hydrochloride in ultrapurewater for 20 min. The surfaces were then washed briefly with ultrapurewater and immediately incubated with 0.1 mg/ml of anamine-functionalized DNA (20 nucleotides) in 10 mM sodium acetate buffer(pH=5). A 0.5 ng/mm² surface concentration of DNA was obtained.

1. Phosphorus-containing polymer, suitable for coating dielectricsurfaces, of the general formula I or II,P(A)_(m)(F)_(n1)(U)_(o1)  (I)P(A)_(m)(UF_(n2))_(o2)  (II) in which P stands for a linear or branched,uncrosslinked homo- or heteropolymeric polymer component selected fromthe group consisting of: i) polyvinyl alcohols, polyvinyl amine,polyallyl amine, polyethylene imine, imides of polymaleicanhydride-alt-methyl vinyl ether or derivatives thereof; ii) linearpolyethylene glycols, polypropylene glycols or derivatives thereof; iii)branched or star-branched polyethylene glycols; iv) polyureas,polyurethanes, polyesters, polycarbonates, polyhydroxycarboxylic acidsor derivatives thereof, which are made up of diols/polyols and/ordiamines/polyamines; v) polysaccharides as cellulose, starch, agarose,dextran, chitosan, hyaluronic acid or derivatives thereof; vi)polypeptides or derivatives thereof which are made of one or morevarious amino acid; and vii) branched polyols based on glycidol; Astands for identical or different phosphorus-containing groups bonded toP, m stands for a number from 3 to 1000, F stands for identical ordifferent functional groups bonded directly or indirectly to P, whichare present in addition to A, n1 stands for a number from 1 to 1000, n2stands for a number from 1 to 100, U stands for identical or different,linear or branched, uncrosslinked oligomeric or polymeric segments, madeup of identical or different monomers, which are bonded to P, o1 standsfor a number from 0 to 1000, o2 stands for a number from 1 to
 1000. 2.Polymer according to claim 1, wherein said polymer containsphosphorus-containing groups A in an amount of from 0.001 to 10 mEq. 3.Polymer according to claim 1, wherein said polymer contains functionalgroups F in an amount of from 0.001 to 20 mEq.
 4. Polymer according toclaim 1, wherein said polymer contains segments U in an amount of from0.001 to 20 mEq.
 5. Polymer according to claim 1, wherein the polymerhas a Mw of from 1000 to 10,000,000 g/mol.
 6. Polymer according to claim1, wherein the polymer component P is a statistical copolymer or blockcopolymer.
 7. Polymer according to claim 1, wherein the polymercomponent P is hydrophilic.
 8. Polymer according to claim 1, whereinsaid polymer contains phosphorus-containing groups A in the form of aspacer carrying from one to six identical or differentphosphorus-containing radicals.
 9. Polymer according to claim 1, whereinsaid polymer contains functional groups F that can form covalent bonds,coordination bonds or take part in biochemical recognition reactions.10. Polymer according to claim 1, wherein said polymer containsfunctional groups F with crosslinkers.
 11. Polymer according to claim 1,wherein the segments U have a Mw of from 100 to 10,000 g/mol. 12.Polymer according to claim 1, wherein the groups or segments U arehydrophilic.
 13. Process for preparing a polymer according to claim 1,wherein the polymer component P is bonded to phosphorus-containing groupA and optionally oligomeric or polymeric segments U, comprising the stepof copolymerizing (A) a monomer containing a phosphorus-containing groupA, or a plurality of identical or different monomers containingidentical or different phosphorus-containing groups A with (B) a monomercontaining a functional group F, or a plurality of identical ordifferent monomers containing identical or different functional groupsF, and (C) optionally, a monomer containing a segment U, or a pluralityof identical or different monomers containing identical or differentsegments U, to form a polymer of the formula I, or with (B′) a monomercontaining a unit (UF₂)_(o2) according to formula II, or a plurality ofidentical or different monomers containing identical or different unitsof the formula (UF₂)_(o2) according to formula II, to form a polymer ofthe formula II.
 14. Process for preparing a polymer according to claim1, comprising the following steps: (i) preparing a polymer, which formsthe polymer component P and carries identical or different functionalgroups that are suitable as functional groups F, (ii) reacting some ofthe functional groups to form identical or differentphosphorus-containing groups A, and (iii) optionally, reacting some ofthe functional groups to form identical or different segments U, whereinstep (iii) can be carried out after, before or together with step (ii),and wherein not all the functional groups are converted in steps (ii)and (iii), and the functional groups that are not converted in steps(ii) and (iii) form the functional groups F of the polymer.
 15. Processaccording to claim 14, wherein some or all of the functional groups thathave not been converted in steps (ii) and (iii) are reacted with one ormore identical or different crosslinkers to form functional groups F.16. Polymer according to claim 1, wherein said polymer containsphosphorus-containing groups A in an amount of from 00.1 to 5 mEq. 17.Polymer according to claim 1, wherein said polymer containsphosphorus-containing groups A in an amount of from 0.1 to 3 mEq. 18.Polymer according to claim 1, wherein said polymer contains functionalgroups F in an amount of from 0.01 to 10 mEq.
 19. Polymer according toclaim 1, wherein said polymer contains functional groups F in an amountof from 0.5 to 10 mEq.
 20. Polymer according to claim 1, wherein saidpolymer contains segments U in an amount of from 0.01 to 10 mEq. 21.Polymer according to claim 1, wherein said polymer contains segments Uin an amount of from 0.5 to 10 mEq.
 22. Polymer according to claim 1,wherein the polymer has a Mw of from 2100 to 1,000,000 g/mol. 23.Polymer according to claim 1, wherein the polymer has a Mw of from 5000to 500,000 g/mol.
 24. Polymer according to claim 1, wherein the polymerhas a Mw of from 5000 to 300,000 g/mol.
 25. Polymer according to claim1, wherein the polymer has a Mw of from 10,000 to 150,000 g/mol. 26.Process for preparing a polymer according to claim 1, comprising thefollowing steps: (i) preparing a polymer, which forms the polymercomponent P and carries identical or different functional groups thatare suitable as functional groups F, said functional groups F beingselected from the group consisting of hydroxyl groups, carboxyl groups,derivatives of carboxyl groups and amine groups, (ii) reacting some ofthe functional groups to form identical or differentphosphorus-containing groups A, and (iii) optionally, reacting some ofthe functional groups to form identical or different segments U, whereinstep (iii) can be carried out after, before or together with step (ii),and wherein not all the functional groups are converted in steps (ii)and (iii), and the functional groups that are not converted in steps(ii) and (iii) form the functional groups F of the polymer.
 27. Thepolymer of claim 1, wherein in polymer component P, the diols/polyolsare polyethylene glycols or polypropylene glycols.
 28. The polymer ofclaim 1, wherein in polymer component P, the diamines/polyamines arejeffamine, polyethylene imines, polyvinyl amine, polyallyl amine orpolyethylene imine.
 29. The polymer of claim 1, wherein in polymercomponent P, the polysaccharide derivatives are hydroxyalkyl derivativesor acid semiesters.
 30. The polymer of claim 1, wherein in polymercomponent P, the polypeptides are selected from the group consisting ofpolylysine, polyphenylalanine-lysine, polyglutamates,polymethylglutamate-glutamate, polyphenylalanine-glutamate, polyserine,polyglycine and polyserine-glycerine.
 31. The polymer of claim 1,wherein in polymer component P, the branched polyols based on glycidolare polyols based on glycidol with a degree of polymerization of 1 to300, a polydispersity index below 1.7, a content of branched units,based on the sum of all monomeric units and determined by ¹³C NMRspectroscopy, of 10 to 33 mol %.